Protein_Domain

Part:BBa_K1390016:Design

Designed by: Stefan Dübel   Group: iGEM14_Braunschweig   (2014-07-04)


mmoX-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1154
    Illegal BamHI site found at 1188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 163
    Illegal BsaI.rc site found at 301
    Illegal BsaI.rc site found at 718


Design Notes

the brick is constructed in the RCF10 standard


Source

This brick was amplified with primers adding a His6-tag to the brick BBa_K1390004

References